Species-specific RNA-FISH probes for yeast identification: evaluation of their specificity and performance
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Sociedade Portuguesa de Microbiologia; Sociedade Portuguesa de Biotecnología; Católica Escola Superior de Biotecnologia, Porto;,
Abstract
The aim of this work was to investigate the possibility of applying the RNA-FISH protocol previously developed by our group for simultaneous analysis of fungi
and bacteria using kingdom-specific probes
for specific detection of various yeast species using previously published species-specific probes. Their specificity
and performance were analysed both in silico and experimentally.