Seed germination in Cistus ladanifer: heat shock, physical dormancy, soil temperatures and significance to natural regeneration

Abstract

Seeds of Cistus ladanifer experience bursts of germination following fires. The effects of heat shock from 10 °C to 150 °C on seed germination were investigated by final germination plus the number of days required for germination to start and finish, and symmetry of cumulative germination. The occurrence of physical dormancy in C. ladanifer seeds was investigated by a variety of methods, including imbibition, scanning electron microscopy (SEM) and light microscopy, and use of dyes. The significance of responses of C. ladanifer seeds to fires was investigated essentially by abstracting existing literature and by using fire effects models and simulations. Parameters of germination were variously affected by heat treatments—positively in the range 80100 °C, negatively above 130 °C. Non-dormancy was consistently found in about 30% of seeds but no evidence was obtained to support the existence of physical dormancy in the dormant fraction of C. ladanifer seeds. Two complementary processes seem to be in place in seeds response to fire. A direct fire-driven increase in germination of virtually all seeds in response to the appropriate heat load produced by fire or, in the absence of such heat loads, the germination of the non-dormant fraction provided that above-ground vegetation burns.