A novel Rhodotorula sp. RNA-FISH probe for the identification of this artwork’s biodeterioration agent

Abstract

Thus, the aim of this study was to design in silico a novel RNA-FISH probe to Rhodotorula sp., that facilitate a precise identification of this yeast, and to evaluate its specificity and experimental performance. The probe designed was a novel 26S RNA probe whose experimental evaluation was done by constructing the fluorescence-signal-response/formamide concentration curve for the target and a non-target yeast (Cryptococcus adeliensis). In silico and experimental results revealed that the Rhodotorula sp. probe is genus-specific. Hence, this study will contribute to an accurate identification of microorganisms involved in the biodeterioration process, advantageous for the selection of proper remediation strategies.