Proteic and molecular markers of parchments
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MicroBiotec15
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The parchments were characterized by microscopy and non-destructive techniques (FTIR-ATR and micro-Raman) and, microsamples were collected in order to perform protein and DNA extraction processes. Results allowed to identify differences at structural level, based on hole patterns and molecular signs. Proteic and DNA profiles, obtained by Native-PAGE and M13-PCR, respectively, allowed to distinguish parchments with different animal origins.
The application of these approaches, using proteic and molecular markers, seems to be a useful tool to complement the study and identification of the animal sources of historical parchments.
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Proteic and molecular markers of parchments,
Cátia Salvador, Catarina Miguel, Ana Branco, António Pereira, António Candeias, A. Teresa Caldeira, MicroBiotec15, Évora, Portugal, Dec. 2015.