High diversity of chickpea Mesorhizobium species isolated in a Portuguese agricultural region

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dc.contributor.authorLaranjo, Marta
dc.contributor.authorMachado, Jorge
dc.contributor.authorYoung, J.Peter W.
dc.contributor.authorOliveira, Solange
dc.date.accessioned2010-09-27T14:47:22Z
dc.date.available2010-09-27T14:47:22Z
dc.date.issued2004
dc.description.abstractChickpea rhizobia isolated from Portuguese soils were assigned to the genus Mesorhizobium by 16S-rDNA sequencing. High species diversity was found within populations of an agricultural region in the south of Portugal.;Besides the expected Mesorhizobium ciceri and M. mediterraneum, some isolates were close to M. loti or M. tianshanense and some formed a clade that may represent a new species. A new PCR-based approach, named direct amplified polymorphic DNA (DAPD) analysis, supported the 16S-based phylogeny. This suggests that this method could be used as a molecular tool to assess genetic relationships. Evaluation of genetic diversity by 16S-rDNA sequence, DAPD and protein profiles showed different levels of heterogeneity in natural populationsen
dc.format.extent123648 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.accesstypelivreen
dc.identifier.authoremailmlaranjo@uevora.pt
dc.identifier.authoremailnd
dc.identifier.authoremailnd
dc.identifier.authoremailismo@uevora.pt
dc.identifier.paginapag 101-107en
dc.identifier.revistaFEMS MICROBIOLOGY ECOLOGYen
dc.identifier.scientificarea221en
dc.identifier.urihttp://hdl.handle.net/10174/2105
dc.identifier.volume48en
dc.language.isoeng
dc.peerreviewednoen
dc.rightsopenAccessen
dc.subjectmesorhizobiumen
dc.subjectdiversityen
dc.titleHigh diversity of chickpea Mesorhizobium species isolated in a Portuguese agricultural regionen
dc.typearticleen

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