Hydrolysis of the phosphoanhydride linkage of cyclic ADP-ribose by the Mn2+-dependent ADP-ribose/CDP-alcohol pyrophosphatase

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dc.contributor.authorCanales, J
dc.contributor.authorFernández, A
dc.contributor.authorRodrigues, JR
dc.contributor.authorFerreira, R
dc.contributor.authorMeireles Ribeiro, J
dc.contributor.authorCabezas, A
dc.contributor.authorCostas, MJ
dc.contributor.authorCameselle, JC
dc.date.accessioned2012-11-20T15:22:00Z
dc.date.available2012-11-20T15:22:00Z
dc.date.issued2009-05
dc.description.abstractCyclic ADP-ribose (cADPR) metabolism in mammals is catalyzed by NAD glycohydrolases (NADases) that, besides forming ADP-ribose, form and hydrolyze the N1-glycosidic linkage of cADPR. Thus far, no cADPR phosphohydrolase was known. We tested rat ADP-ribose/CDP-alcohol pyrophosphatase (ADPRibase-Mn) and found that cADPR is an ADPRibase-Mn ligand and substrate. ADPRibase-Mn activity on cADPR was 65-fold less efficient than on ADP-ribose, the best substrate. This is similar to the ADP-ribose/cADPR formation ratio by NADases. The product of cADPR phosphohydrolysis by ADPRibase-Mn was N1-(5-phosphoribosyl)-AMP, suggesting a novel route for cADPR turnover.por
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dc.identifier.authoremailraf@uevora.pt
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dc.identifier.citationCanales J, Fernández A, Rodrigues JR, Ferreira R, Meireles Ribeiro J, Cabezas A, Costas MJ, Cameselle JC (2009) - Hydrolysis of the phosphoanhydride linkage of cyclic ADP-ribose by the Mn2+-dependent ADP-ribose/CDP-alcohol pyrophosphatase, FEBS Letters 583, 1593–1598 (doi:10.1016/j.febslet.2009.04.023)por
dc.identifier.scientificarea365por
dc.identifier.sharewithICAAMpor
dc.identifier.urihttp://www.febsletters.org/article/S0014-5793(09)00297-X/abstract
dc.identifier.urihttp://hdl.handle.net/10174/5804
dc.language.isoporpor
dc.peerreviewedyespor
dc.rightsopenAccesspor
dc.subjectCyclic ADP-ribosepor
dc.subjectADP-ribosepor
dc.subjectPyrophosphatasepor
dc.subjectPhosphoribosyl-AMPpor
dc.subjectHistidine biosynthesispor
dc.subjectImmune signalingpor
dc.titleHydrolysis of the phosphoanhydride linkage of cyclic ADP-ribose by the Mn2+-dependent ADP-ribose/CDP-alcohol pyrophosphatasepor
dc.typearticlepor

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