Monoclonal antibody to Olive latent virus 1

Abstract

Olive is a crop of high economic importance in Portugal. Its susceptibility to virus infections justifies research leading to accurate virus detection. Two necroviruses mechanically transmissible to herbaceous indicator plants have been isolated from symptomatic olive trees: Olive latent virus 1 (OLV-1) and Tobacco necrosis virus D (TNV-D). OLV-1 was also isolated from citrus trees in Turkey and from tulips in Japan. In this study, we attempted to prepare a monoclonal antibody (MAb) specific to OLV-1, which could be used in ELISA for accurate diagnostics of this virus.