Precision and selection of extraction methods of aphelenchid nematodes from maritime pine wood, Pinus pinaster L.

Abstract

Four extraction methods for Bursaphelenchus xylophilus and other aphelenchid nematodes were compared on the number of nematodes per gram recovered, and on the precision of the mean number of nematodes per gram of pine wood. The number of nematodes per gram recovered by each method, in addition to its inherent shortcomings when the actual number of nematodes is unknown, failed to provide clear rankings among the extraction methods. The precision of the mean number of nematodes per gram did provide clear guidelines for selection. Selection of the method may use prior knowledge about the range of nematodes to be expected or the independence of precision from the mean number of nematodes.